Journal: Frontiers in Immunology

Article Title: Aqueous Artemisia argyi extract mitigates acute lung injury in association with coordinated alterations in gut microbiota, metabolic homeostasis, and pulmonary inflammatory gene expression

doi: 10.3389/fimmu.2026.1770675

Figure Lengend Snippet: AEAA pretreatment is associated with reduced pathological features of LPS-induced acute lung injury in mice. (A) Experimental design of the LPS-induced acute lung injury (ALI) model. (B) Histopathological evaluation of lung tissues. Representative H&E-stained sections from different treatment groups at 20× and 40× magnification show alveolar wall thickening, inflammatory cell infiltration, hemorrhage, and edema in LPS-treated mice. AEAA treatment dose-dependently ameliorated these pathological alterations (red boxed areas). (C) Wet-to-dry (W/D) lung weight ratio. (D) Lung injury score. (E–G) Pro-inflammatory cytokines in serum and BALF.IL-1β (E) , IL-6 (F) , and IL-10 (G) levels in serum and BALF. (H) TNF-α levels in serum and BALF. (I) Myeloperoxidase (MPO) activity. Data are presented as mean ± SEM. n = 5 per group. Statistical analysis was performed using one-way ANOVA followed by post hoc tests. * P < 0.05 , ** P < 0.01 , *** P < 0.001 , **** P < 0.0001 .

Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kits for mouse IL-1β (Cat. #EK201B), IL-6 (Cat. #EK206), IL-10 (Cat. #EK210), TNF-α (Cat. #EK282), and myeloperoxidase (MPO) (Cat. #EK2133) were obtained from Multisciences (Lianke) Biotech Co., Ltd. (Hangzhou, China).

Techniques: Staining, Activity Assay

Journal: International Journal of Molecular Sciences

Article Title: NETosis-Related Biomarkers in Systemic Lupus Erythematosus, Rheumatoid Arthritis, Psoriatic Arthritis and Ankylosing Spondylitis: A Comparative Study

doi: 10.3390/ijms262412127

Figure Lengend Snippet: Concentration of specific NETosis biomarkers, citrullinated histone H3 (H3cit) ( A ) and myeloperoxidase (MPO) ( B ), in plasma of patients with rheumatic diseases and healthy subjects. The data are presented as boxplots similar to . The significance of differences was assessed using ANCOVA, accounting for sex, age and disease duration as covariates, with a post hoc test. Only significant p -values that passed Tukey’s correction for multiple comparisons are presented.

Article Snippet: MPO concentration was measured using the Uncoated Human MPO (Myeloperoxidase) ELISA Kit (Cat. # E-UNEL-H0048, Elabscience, Houston, TX, USA).

Techniques: Concentration Assay, Clinical Proteomics

Journal: BMC Musculoskeletal Disorders

Article Title: Neutrophil extracellular traps induce the bone erosion of gout

doi: 10.1186/s12891-022-06115-w

Figure Lengend Snippet: MSU crystals induce the formation of NETs in vivo. a The concentration of DNA, NE, MPO in the MSU group was higher than the NC group on days 1, 7 and 14. * P < 0.05, ** P < 0.01, *** P < 0.001 as determined by t test ( n = 3). b The immunofluorescence of the joint flushing fluid (× 20) The formation of NETs (MPO + H3 + DNA + ) in group MSU was significantly higher than NC groups on days 1, 7 and 14. c The histopathology of tibia (× 100). There was slight inflammatory infiltration in the cartilage on 1 day, while that was relieved at 7 day and 14 days in the MSU group. d IL-1β、TNF-αwere measured with ELISA. The level of two of them were higher than NC group at 24 h, on the day 7 and 14, the comparison was no longer statistically significant. This result was consistent with c

Article Snippet: The levels of IL-1β(Dakewe Biotech, 1310122), TNF-α(Dakewe Biotech, 1317202), NE (Cusabio, CSB-E08847r), and MPO (Cusabio, CSB-E08722R) were detected using ELISA kit according to the manufacturer's protocol.

Techniques: In Vivo, Concentration Assay, Immunofluorescence, Histopathology, Enzyme-linked Immunosorbent Assay, Comparison